to study the molecular and epigenetic role of SMYD1 in sarcomeric renewal and regeneration in zebrafish and mammalian cardiomyocytes.

Due to our recent findings on the important role of methyltransferase SMYD1 on the methylation of sarcomeric Myosin, its assembly and homeostasis during cardiac development (Diofano et al., 2024), we will now focus this research project on the impact of SMYD1 on (1) Myosin methylation, cardiomyocyte de-dedifferentiation, sarcomeric breakdown, and functional cardiomyocyte re-differentiation during the regeneration of the adult zebrafish heart. Secondly, we will aim to (2) translate our recent zebrafish SMYD1 findings into the mammalian/human system by investigating the role SMYD1 on MYOSIN methylation, sarcomeric assembly and cardiomyocyte function in human iPSC-derived cardiomyocytes. The long-term goal of this project is to foster regenerative strategies as therapeutic options for acute or chronic myocardial injury.
The position offers the opportunity to work on a dynamic and innovative project employing cutting-edge technologies. These include in vitro studies (iPSC-derived cardiomyocytes) and in vivo (zebrafish) functional genomics. Advanced methods such as CRISPR/Cas9 gene editing, transgenesis, molecular biology, biochemistry, and high-resolution microscopy will be key components of the research. Additionally, omics-based approaches, such as transcriptomics, proteomics, and interactomics, will be used to generate comprehensive datasets. This research will focus on analyzing mutant zebrafish and human cardiomyocyte cell lines to explore molecular pathways involved in cardiac development, regeneration, and aging.
The successful candidate will join a collaborative, international research environment supported by state-of-the-art facilities. The role provides exceptional training opportunities, not only in technical and experimental methodologies but also in scientific communication and career development.