in the field of "Pancreatic Development, Stem Cell Differentiation and Cancer".
You will take part in our PDAC modelling efforts based on an approach to direct human pluripotent stem cells (hPSCs) towards pancreatic organoids (POs) resembling acinar and ductal progeny. In the research project, this new methodology will be combined with personalized in vitro models for dysplasia and cancer onset in the pancreas. You will be working with genetically engineered human embryonic and patient-derived induced pluripotent stem cells and implement state-of-the-art technologies, including 3D culture systems, Crispr/Cas9 genome editing, single cell transcriptomics, ATAC-seq, and real-time imaging, to analyze molecular processes of pancreatic cancer initiation and early lesion formation. In addition, we plan to apply cellular barcoding for lineage tracing at single-cell resolution to dissect the cell type of origin in PDAC development in response to specific oncogenic Events.
What we offer you:
You will join a young, dynamic and enthusiastic team in an inspiring research field by daily interaction with clinical and academic research groups in Ulm. The lab offers a strong and successful track record in embryonic stem cell differentiation and basic pancreatic cancer research. Education of students within the framework of our International Graduate School in Molecular Medicine Ulm (IGradU) (https://www.uni-ulm.de/en/einrichtungen/mm/) would be desirable.
Applicants should by highly motivated and should have recently acquired a Master’s degree in molecular medicine, biology or related disciplines. We are looking for one PhD student and one postdoctoral fellow with hands-on experience in mammalian cell culture and standard molecular biology techniques (qPCR, western blot, DNA cloning, etc.). Previous experience in FACS, immunostaining, or microscopic imaging would be desirable, additional bioinformatics skills or knowledge of basic computational tools would be outstanding. Previous first authorship publications are mandatory, several ones in case of the postdoc position.
In addition to being an enthusiastic scientist, we are seeking individuals who will collaborate with an international group of researchers to work on this highly complex and interdisciplinary topic.
Level of employment: full-time/part-time